Infection courses, virological features and IFN-α responses of HBV genotypes in cell culture and animal models.

ABSTRACT:

Background & aims

HBV consists of 9 major genotypes (A to I), 1 minor strain (designated J) and multiple subtypes, which may be associated with different clinical characteristics. As only cell lines expressing genotype D3 have been established, herein, we aimed to establish stable cell lines producing high-titer cell culture-generated HBV (HBVcc) of different genotypes and to explore their infectivity, virological features and responses to treatment.

Methods

Stable cell lines producing high titers of HBV genotype A2, B2, C1, E, F1b and H were generated by transfecting plasmids containing a replication-competent 1.3x length HBV genome and an antibiotic marker into HepG2 cells that can support HBV replication. Clones with the highest levels of HBV DNA and/or HBeAg were selecte

SUBMITTER: Zhang M 

PROVIDER: S-EPMC8604785 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature