A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation.

ABSTRACT: Three-dimensional aggregate-suspension culture is a potential biomanufacturing method to produce a large number of human induced pluripotent stem cells (hiPSCs); however, the use of expensive growth factors and method-induced mechanical stress potentially result in inefficient production costs and difficulties in preserving pluripotency, respectively. Here, we developed a simple, miniaturized, dual-compartment dialysis-culture device based on a conventional membrane-culture insert with deep well plates. The device improved cell expansion up to approximately ~3.2 to 4×10⁷ cells/mL. The high-density expansion was supported by reduction of excessive shear stress and agglomeration mediated by the addition of the functional polymer FP003. The results revealed accumulation of several

SUBMITTER: Torizal FG 

PROVIDER: S-EPMC8604949 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature