Self-organization of human dorsal-ventral forebrain structures by light induced SHH.

ABSTRACT: Organizing centers secrete morphogens that specify the emergence of germ layers and the establishment of the body's axes during embryogenesis. While traditional experimental embryology tools have been instrumental in dissecting the molecular aspects of organizers in model systems, they are impractical in human in-vitro model systems to dissect the relationships between signaling and fate along embryonic coordinates. To systematically study human embryonic organizer centers, we devised a collection of optogenetic ePiggyBac vectors to express a photoactivatable Cre-loxP recombinase, that allows the systematic induction of organizer structures by shining blue-light on human embryonic stem cells (hESCs). We used a light stimulus to geometrically confine SHH expression in neuralizing hESCs. Thi

SUBMITTER: De Santis R 

PROVIDER: S-EPMC8604999 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature