Gene expression analysis of human induced pluripotent stem cells cryopreserved by vitrification using StemCell Keep.

ABSTRACT: In recent years, regenerative medicine research using human somatic and induced pluripotent stem cells has advanced considerably, promoting clinical applications. However, it is essential that these cells are cryopreserved safely and effectively. Most cryopreservation solution agents contain dimethyl sulfoxide (DMSO), which exhibits strong toxicity and can potentially promote cell differentiation. Hence, it is important to explore substitutes for DMSO in cryoprotectant solutions. One such alternative is StemCell Keep (SCK), a DMSO-free solution that has been reported to effectively cryopreserve human induced pluripotent stem cells (hiPS cells). To clarify the effect of cryopreservation agents on cells, DNA microarray analysis is useful, as it can identify a large number of gene expression

SUBMITTER: Ota A 

PROVIDER: S-EPMC8605251 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature