The effect of charged residue substitutions on the thermodynamics of protein-surface interactions.

ABSTRACT: The interactions of proteins with surfaces are important in both biological processes and biotechnologies. In contrast to decades of study regarding the biophysics of proteins in bulk solution, however, our mechanistic understanding of the biophysics of proteins interacting with surfaces remains largely qualitative. In response, we have set to explore quantitatively the thermodynamics of protein-surface interactions. In this work, we explore systematically the role of electrostatics in modulating the interaction between proteins and charged surfaces. In particular, we use electrochemistry to explore the extent to which a macroscopic, hydroxyl-coated surface held at a slightly negative potential affects the folding thermodynamics of surface-attached protein variants with different compositi

SUBMITTER: Ortega G 

PROVIDER: S-EPMC8605371 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature