The genotypic and phenotypic impact of hypoxia microenvironment on glioblastoma cell lines.

ABSTRACT:

Background

Glioblastoma is a fatal brain tumour with a poor patient survival outcome. Hypoxia has been shown to reprogram cells towards a stem cell phenotype associated with self-renewal and drug resistance properties. Activation of hypoxia-inducible factors (HIFs) helps in cellular adaptation mechanisms under hypoxia. Similarly, miRNAs are known to be dysregulated in GBM have been shown to act as critical mediators of the hypoxic response and to regulate key processes involved in tumorigenesis.

Methods

Glioblastoma (GBM) cells were exposed to oxygen deprivation to mimic a tumour microenvironment and different cell aspects were analysed such as morphological changes and gene expression of miRNAs and survival genes known to be associated with tumorigenesis.

Results

It

SUBMITTER: Macharia LW 

PROVIDER: S-EPMC8605580 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature