The Tight Junction Protein ZO-1 Is Dispensable for Barrier Function but Critical for Effective Mucosal Repair.

ABSTRACT:

Backgrounds & aims

Increased permeability is implicated in the pathogenesis of intestinal disease. In vitro and in vivo studies have linked down-regulation of the scaffolding protein ZO-1, encoded by the TJP1 gene, to increased tight junction permeability. This has not, however, been tested in vivo. Here, we assessed the contributions of ZO-1 to in vivo epithelial barrier function and mucosal homeostasis.

Methods

Public Gene Expression Omnibus data sets and biopsy specimens from patients with inflammatory bowel disease (IBD) and healthy control individuals were analyzed. Tjp1^f/f;vil-Cre^Tg mice with intestinal epithelial-specific ZO-1 knockout (ZO-1^KO.IEC) mice and Tjp1^f/f mice littermates without Cre expression were studied using c

SUBMITTER: Kuo WT 

PROVIDER: S-EPMC8605999 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature