Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing.

ABSTRACT: The bone marrow microenvironment is composed primarily of immune and stromal cells that play important roles in fracture healing. Although immune cells have been identified in mouse bone marrow, variations in their numbers and type during the fracture healing process remain poorly defined. In this study, single-cell RNA sequencing was used to identify immune cells in fracture tissues, including neutrophils, monocytes, T cells, B cells, and plasma cells. The number of B cells decreased significantly in the early stage of fracture healing. Furthermore, B cells in mice fracture models decreased significantly during the epiphyseal phase and then gradually returned to normal during the epiphyseal transformation phase of fracture healing. The B-cell pattern was opposite to that of bone formation

SUBMITTER: Zhang H 

PROVIDER: S-EPMC8606664 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature