Utilization of a 3-D tissue engineered model to investigate the effects of perfusion on gynecologic cancer biology.

ABSTRACT: Among gynecologic malignancies, ovarian cancer (OC) has the poorest survival rate, and its clinical management remains challenging due to the high rate of recurrence and chemoresistance. Improving survival for these patients is critical, although this requires the ability to translate preclinical studies to actual patient care: bench to bedside and back. Our objective was to develop a preclinical model that accurately represents tumor biology and its microenvironment. We utilized SKOV-3, OVCAR-8, and CS-99 cell lines to show that this model was suitable for in vitro assessment of cell proliferation. We tested OC cells independently and in co-culture with cancer associated fibroblasts (CAFs) or immune cells. Additionally, we used patient-derived ovarian carcinoma and carcinosarcoma samples

SUBMITTER: Martinez A 

PROVIDER: S-EPMC8606733 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature