An Analysis of Metabolic Changes in the Retina and Retinal Pigment Epithelium of Aging Mice.

ABSTRACT:

Purpose

The purpose of this study was to present our hypothesis that aging alters metabolic function in ocular tissues. We tested the hypothesis by measuring metabolism in aged murine tissues alongside retinal responses to light.

Methods

Scotopic and photopic electroretinogram (ERG) responses in young (3-6 months) and aged (23-26 months) C57Bl/6J mice were recorded. Metabolic flux in retina and eyecup explants was quantified using U-13C-glucose or U-13C-glutamine with gas chromatography-mass spectrometry (GC-MS), O2 consumption rate (OCR) in a perifusion apparatus, and quantifying adenosine triphosphatase (ATP) with a bioluminescence assay.

Results

Scotopic and photopic ERG responses were reduced in aged mice. Glucose metabolism, glutamine metabolism, OCR, and ATP poo

SUBMITTER: Tsantilas KA 

PROVIDER: S-EPMC8606884 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature