Establishment and characterization of prostate organoids from treatment-naive patients with prostate cancer.

ABSTRACT: Three-dimensional (3D) organoid culture systems are emerging as potential reliable tools to investigate basic developmental processes of human disease, especially cancer. The present study used established and modified culture conditions to report successful generation and characterization of patient-derived organoids from fresh primary tissue specimens of patients with treatment-naïve prostate cancer (PCa). Fresh tissue specimens were collected, digested enzymatically and the resulting cell suspensions were plated in a 3D environment using Matrigel as an extracellular matrix. Previously established 12-factor medium for organoid culturing was modified to create a minimal 5-factor medium. Organoids and corresponding tissue specimens were characterized using transcriptomic analysis, immunofl

SUBMITTER: Cheaito K 

PROVIDER: S-EPMC8607232 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature