A Method to Study de novo Formation of Chromatin Domains.

ABSTRACT: The organization and structure of chromatin domains are unique to individual cell lineages. Their misregulation might lead to a loss in cellular identity and/or disease. Despite tremendous efforts, our understanding of the formation and propagation of chromatin domains is still limited. Chromatin domains have been studied under steady-state conditions, which are not conducive to following the initial events during their establishment. Here, we present a method to inducibly reconstruct chromatin domains and follow their re-formation as a function of time. Although, first applied to the case of PRC2-mediated repressive chromatin domain formation, it could be easily adapted to other chromatin domains. The modification of and/or the combination of this method with genomics and imaging technolo

SUBMITTER: Oksuz O 

PROVIDER: S-EPMC8607434 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature