Comparison of fecal and oral collection methods for studies of the human microbiota in two Iranian cohorts.

ABSTRACT:

Background

To initiate fecal and oral collections in prospective cohort studies for microbial analyses, it is essential to understand how field conditions and geographic differences may impact microbial communities. This study aimed to investigate the impact of fecal and oral sample collection methods and room temperature storage on collection samples for studies of the human microbiota.

Results

We collected fecal and oral samples from participants in two Iranian cohorts located in rural Yazd (n = 46) and urban Gonbad (n = 38) and investigated room temperature stability over 4 days of fecal (RNAlater and fecal occult blood test [FOBT] cards) and comparability of fecal and oral (OMNIgene ORAL kits and Scope mouthwash) collection methods. We calculated interclass correlation c

SUBMITTER: Wu Z 

PROVIDER: S-EPMC8607576 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature