Six1 promotes skeletal muscle thyroid hormone response through regulation of the MCT10 transporter.

ABSTRACT:

Background

The Six1 transcription factor is implicated in controlling the development of several tissue types, notably skeletal muscle. Six1 also contributes to muscle metabolism and its activity is associated with the fast-twitch, glycolytic phenotype. Six1 regulates the expression of certain genes of the fast muscle program by directly stimulating their transcription or indirectly acting through a long non-coding RNA. We hypothesized that additional mechanisms of action of Six1 might be at play.

Methods

A combined analysis of gene expression profiling and genome-wide location analysis data was performed. Results were validated using in vivo RNA interference loss-of-function assays followed by measurement of gene expression by RT-PCR and transcriptional reporter assays.

SUBMITTER: Girgis J 

PROVIDER: S-EPMC8607597 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature