Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12.

ABSTRACT:

Background

Cellulase plays a key role in converting cellulosic biomass into fermentable sugar to produce chemicals and fuels, which is generally produced by filamentous fungi. However, most of the filamentous fungi obtained by natural breeding have low secretory capacity in cellulase production, which are far from meeting the requirements of industrial production. Random mutagenesis combined with adaptive laboratory evolution (ALE) strategy is an effective method to increase the production of fungal enzymes.

Results

This study obtained a mutant of Trichoderma afroharzianum by exposures to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), Ethyl Methanesulfonate (EMS), Atmospheric and Room Temperature Plasma (ARTP) and ALE with high sugar stress. The T. afroharzianum mutant MEA-12

SUBMITTER: Peng ZQ 

PROVIDER: S-EPMC8607671 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature