Accurate and reproducible diagnosis of peanut allergy using epitope mapping.

ABSTRACT:

Background

Accurate diagnosis of peanut allergy is a significant clinical challenge. Here, a novel diagnostic blood test using the peanut bead-based epitope assay ("peanut BBEA") was developed utilizing the LEAP cohort and then validated using two independent cohorts.

Methods

The development of the peanut BBEA diagnostic test followed the National Academy of Medicine's established guidelines with discovery performed on 133 subjects from the non-interventional arm of the LEAP trial and an independent validation performed on 82 subjects from the CoFAR2 and 84 subjects from the POISED study. All samples were analyzed using the peanut BBEA methodology, which measures levels of IgE to two Ara h 2 sequential (linear) epitopes and compares their combination to a threshold pre-speci

SUBMITTER: Suarez-Farinas M 

PROVIDER: S-EPMC8607840 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature