Transcriptional reprogramming by oxidative stress occurs within a predefined chromatin accessibility landscape.

ABSTRACT: Reactive oxygen species (ROS) are important signaling molecules in many physiological processes, yet excess ROS leads to cell damage and can lead to pathology. Accordingly, cells need to maintain tight regulation of ROS levels, and ROS-responsive transcriptional reprogramming is central to this process. Although it has long been recognized that oxidative stress leads to rapid, significant changes in gene expression, the impact of oxidative stress on the underlying chromatin accessibility landscape remained unclear. Here, we asked whether ROS-responsive transcriptional reprogramming is accompanied by reprogramming of the chromatin environment in MCF7 human breast cancer cells. Using a time-course exposure to multiple inducers of oxidative stress, we determined that the widespread ROS-respon

SUBMITTER: Levings DC 

PROVIDER: S-EPMC8608001 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature