DNA Damage-Induced Phosphorylation of Histone H2A at Serine 15 Is Linked to DNA End Resection.

ABSTRACT: The repair of DNA double-strand breaks (DSBs) occurs in chromatin, and several histone posttranslational modifications have been implicated in the process. Modifications of the histone H2A N-terminal tail have also been linked to DNA damage response, through acetylation or ubiquitination of lysine residues that regulate repair pathway choice. Here, we characterize a new DNA damage-induced phosphorylation on chromatin, at serine 15 of H2A in yeast. We show that this SQ motif functions independently of the classical S129 C-terminal site (γ-H2A) and that mutant-mimicking constitutive phosphorylation increases cell sensitivity to DNA damage. H2AS129ph is induced by Tel1^ATM and Mec1^ATR, and the loss of Lcd1^ATRIP or Mec1 signaling decreases γ-H2A spreading distal

SUBMITTER: Ahmad S 

PROVIDER: S-EPMC8608016 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature