Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting.

ABSTRACT: The emergence of the COVID-19 pandemic resulted in an unprecedented need for RT-qPCR-based molecular diagnostic testing, placing a strain on the supply chain and the availability of commercially available PCR testing kits and reagents. The effect of limited molecular diagnostics-related supplies has been felt across the globe, disproportionally impacting molecular diagnostic testing in developing countries where acquisition of supplies is limited due to availability. The increasing global demand for commercial molecular diagnostic testing kits and reagents has made standard PCR assays cost prohibitive, resulting in the development of alternative approaches to detect SARS-CoV-2 in clinical specimens, circumventing the need for commercial diagnostic testing kits while mitigating the high-dem

SUBMITTER: Ferreira BIDS 

PROVIDER: S-EPMC8608302 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature