Brucella abortus S19 GFP-tagged vaccine allows the serological identification of vaccinated cattle.

ABSTRACT: Bovine brucellosis induces abortion in cows, produces important economic losses, and causes a widely distributed zoonosis. Its eradication was achieved in several countries after sustained vaccination with the live attenuated Brucella abortus S19 vaccine, in combination with the slaughtering of serologically positive animals. S19 induces antibodies against the smooth lipopolysaccharide (S-LPS), making difficult the differentiation of infected from vaccinated bovines. We developed an S19 strain constitutively expressing the green fluorescent protein (S19-GFP) coded in chromosome II. The S19-GFP displays similar biological characteristics and immunogenic and protective efficacies in mice to the parental S19 strain. S19-GFP can be distinguished from S19 and B. abortus field strains by fluores

SUBMITTER: Chacon-Diaz C 

PROVIDER: S-EPMC8608319 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature