Single cell analysis of DNA in more than 10,000 individual sperm from men with abnormal reproductive outcomes.

ABSTRACT:

Purpose

This pilot study sought to (1) validate the use of a novel technology for single-sperm-cell genome sequencing (Sperm-seq) in infertile men who may not have optimal quantity or quality of sperm for genomic analysis and (2) compare these results to fertile donors.

Methods

Infertile men undergoing IVF with female partners with a previous history of failed fertilization with ICSI (FF) or poor blastulation of embryos (PB) were recruited from a large IVF center. Sperm-seq was used to analyze thousands of individual sperm and was carried out at an affiliated university research institute. Global aneuploidy rate, crossover locations, and crossover frequencies were assessed in the infertile population, and compared with a control group of 20 fertile donors, which were analyze

SUBMITTER: Leung AQ 

PROVIDER: S-EPMC8608944 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature