Different CO₂ settings (6.0% vs 7.0%) do have an impact on extracellular pH of culture medium (pHe) and euploidy rates rather than on blastocyst development: a sibling oocyte study.

ABSTRACT:

Objective

To determine whether euploidy rates and blastocyst development differ in a continuous culture medium under different CO₂ concentrations.

Design and method

A single-center retrospective study was performed from July 2018 to October 2019 including 44 fresh cycles with at least four fresh mature oocytes (MII) without severe male factor infertility. Sibling MII were injected and cultured in Global®Total®LP under 6.0% (pHe = 7.374 ± 0.014) or 7.0% (pHe = 7.300 ± 0.013) CO₂, 5.0% O₂, and 89.0% or 88.0% N₂. Analyzed variables were normally fertilized oocytes (2PN), cleavage rate, blastulation rate on day 5/2PN, usable blastocyst (blastocysts biopsied/2PN), and euploidy rates. Blastocyst's trophectoderm biopsy was performed on da

SUBMITTER: Abdala A 

PROVIDER: S-EPMC8609041 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature