Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription.

ABSTRACT: Nuclear transfer systems represent the efficient means to reprogram a cell and in theory provide a basis for investigating the development of endangered species. However, conventional nuclear transfer using oocytes of laboratory animals does not allow reprogramming of cross-species nuclei owing to defects in cell divisions and activation of embryonic genes. Here, we show that somatic nuclei transferred into mouse four-cell embryos arrested at the G2/M phase undergo reprogramming toward the embryonic state. Remarkably, genome-wide transcriptional reprogramming is induced within a day, and ZFP281 is important for this replication-free reprogramming. This system further enables transcriptional reprogramming of cells from Oryx dammah, now extinct in the wild. Thus, our findings indicate

SUBMITTER: Tomikawa J 

PROVIDER: S-EPMC8609233 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature