Sensitive sandwich-type electrochemical SARS-CoV‑2 nucleocapsid protein immunosensor.

ABSTRACT: A sensitive and fast sandwich-type electrochemical SARS-CoV‑2 (COVID-19) nucleocapsid protein immunosensor was prepared based on bismuth tungstate/bismuth sulfide composite (Bi₂WO₆/Bi₂S₃) as electrode platform and graphitic carbon nitride sheet decorated with gold nanoparticles (Au NPs) and tungsten trioxide sphere composite (g-C₃N₄/Au/WO₃) as signal amplification. The electrostatic interactions between capture antibody and Bi₂WO₆/Bi₂S₃ led to immobilization of the capture nucleocapsid antibody. The detection antibody was then conjugated to g-C₃N₄/Au/WO₃ via the affinity of amino-gold. After physicochemically characterization via transmis

SUBMITTER: Karaman C 

PROVIDER: S-EPMC8609265 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature