A Histoplasma capsulatum Lipid Metabolic Map Identifies Antifungal Targets.

ABSTRACT: Lipids play a fundamental role in fungal cell biology, being essential cell membrane components and major targets of antifungal drugs. A deeper knowledge of lipid metabolism is key for developing new drugs and a better understanding of fungal pathogenesis. Here, we built a comprehensive map of the Histoplasma capsulatum lipid metabolic pathway by incorporating proteomic and lipidomic analyses. We performed genetic complementation and overexpression of H. capsulatum genes in Saccharomyces cerevisiae to validate reactions identified in the map and to determine enzymes responsible for catalyzing orphan reactions. The map led to the identification of both the fatty acid desaturation and the sphingolipid biosynthesis pathways as targets for drug development. We found that the sphingolipid biosy

SUBMITTER: Zamith-Miranda D 

PROVIDER: S-EPMC8609360 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature