The "PepSAVI-MS" Pipeline for Natural Product Bioactive Peptide Discovery.

ABSTRACT: The recent increase in extensively drug-resistant bacterial pathogens and the associated increase of morbidity and mortality demonstrate the immediate need for new antibiotic backbones with novel mechanisms of action. Here, we report the development of the PepSAVI-MS pipeline for bioactive peptide discovery. This highly versatile platform employs mass spectrometry and statistics to identify bioactive peptide targets from complex biological samples. We validate the use of this platform through the successful identification of known bioactive peptides from a botanical species, Viola odorata. Using this pipeline, we have widened the known antimicrobial spectrum for V. odorata cyclotides, including antibacterial activity of cycloviolacin O2 against A. baumannii. We further demonstrate the broa

SUBMITTER: Kirkpatrick CL 

PROVIDER: S-EPMC8609470 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature