DNAJB6b is Downregulated in Synucleinopathies.

ABSTRACT:

Background

α-synuclein (α-syn) aggregation contributes to the progression of multiple neurodegenerative diseases. We recently found that the isoform b of the co-chaperone DNAJB6 is a strong suppressor of α-syn aggregation in vivo and in vitro. However, nothing is known about the role of the endogenous isoform b of DNAJB6 (DNAJB6b) in health and disease, due to lack of specific antibodies.

Objective

Here we generated a novel anti-DNAJB6b antibody to analyze the localization and expression of this isoform in cells, in tissue and in clinical material.

Methods

To address this we used immunocytochemistry, immunohistochemistry, as well as a novel quantitative DNAJB6 specific ELISA method.

Results

The endogenous protein is mainly expressed in the cytoplasm and in neur

SUBMITTER: Folke J 

PROVIDER: S-EPMC8609689 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature