Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes.

ABSTRACT:

Objectives

Increased levels of serum Immunoglobulin-E (IgE) and different genetic variants of cytokines are common biochemical manifestation in Allergy. The current study was aimed to study the association of IgE and different variants of Interleukin-4 (IL-4), and Interleukin-13 (IL-13) genes with different kind of allergies.

Methods

A pre-tested questionnaire was used to collect all the dietary, life style and clinical details by a trained staff. A blood sample of 2 ml each was collected in coagulated and anti-coagulated vials. DNA and serum samples were extracted and stored until further use. Serum IgE were estimated by ELISA while as the genotypic analysis was done by PCR-RFLP methods.

Results

Statistically a significant difference of serum IgE levels were observed

SUBMITTER: Qurashi TA 

PROVIDER: S-EPMC8609820 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature