Microbiota in mesenteric adipose tissue from Crohn's disease promote colitis in mice.

ABSTRACT:

Background

Mesenteric adipose tissue (mAT) hyperplasia, known as creeping fat is a pathologic characteristic of Crohn's disease (CD). The reserve of creeping fat in surgery is associated with poor prognosis of CD patients, but the mechanism remains unknown.

Methods

Mesenteric microbiome, metabolome, and host transcriptome were characterized using a cohort of 48 patients with CD and 16 non-CD controls. Multidimensional data including 16S ribosomal RNA gene sequencing (16S rRNA), host RNA sequencing, and metabolome were integrated to reveal network interaction. Mesenteric resident bacteria were isolated from mAT and functionally investigated both in the dextran sulfate sodium (DSS) model and in the Il10 gene-deficient (Il10^-/-) mouse colitis model to validate their

SUBMITTER: He Z 

PROVIDER: S-EPMC8609859 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature