The thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses.

ABSTRACT:

Background

Arabidopsis protein disulfide isomerase 1 (AtPDI1) has been demonstrated to have disulfide isomerase activity and to be involved in the stress response. However, whether the anti-stress function is directly related to the activities of thiol-disulfide exchange remains to be elucidated.

Results

In the present study, encoding sequences of AtPDI1 of wild-type (WT) and double-cysteine-mutants were transformed into an AtPDI1 knockdown Arabidopsis line (pdi), and homozygous transgenic plants named pdi-AtPDI1, pdi-AtPDI1_m1 and pdi-AtPDI1_m2 were obtained. Compared with the WT and pdi-AtPDI1, the respective germination ratios of pdi-AtPDI1_m1 and pdi-AtPDI1_m2 were significantly lower under abiotic stresses and exogenous ABA treatm

SUBMITTER: Lu Y 

PROVIDER: S-EPMC8609882 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature