Heat shock enhances outer-membrane vesicle release in Bordetella spp.

ABSTRACT: Pertussis, also known as whooping cough, is caused by the Gram-negative bacterium Bordetella pertussis, an obligate human pathogen. Despite high vaccination rates in high-income countries, resurgence of pertussis cases is an occurring problem that urges the necessity of developing an improved vaccine. Likewise, the efficacy of vaccines for Bordetella bronchiseptica, which causes similar disease in pigs and companion animals, is debatable. A promising approach for novel vaccines is the use of outer membrane vesicles (OMVs). However, spontaneous OMV (sOMV) release by Bordetella spp. is too low for cost-effective vaccine production. Therefore, we investigated the influence of growth in various media commonly used for culturing Bordetella in the Bvg⁺, i.e

SUBMITTER: de Jonge EF 

PROVIDER: S-EPMC8610307 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature