Identification of Genes Potentially Associated with Melanoma Tumorigenesis Through Co-Expression Network Analysis.

ABSTRACT:

Background

Melanoma is one of the most malignant and aggressive skin tumors, and its incidence is increasing worldwide. However, few studies have investigated the process of tumorigenesis from normal skin to melanoma.

Methods

Several bioinformatics analyses, including GEO databases, Oncomine database, TCGA database, STRING, MCODE and cytoHubba plug-ins, GEPIA, TIMER and TRRUST and DGIdb, were performed to disclose the hub genes and immunology implicated in primary melanoma tumorigenesis. Finally, immunohistochemistry (IHC) and quantitative real-time PCR (qPCR) were used to validate the results of bioinformatics analysis in vitro.

Results

A total of 295 overlapping DEGs (ODEGs) (157 upregulated and 138 downregulated) and 9 hub genes were identified between primary mela

SUBMITTER: Xuan X 

PROVIDER: S-EPMC8610383 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature