BRAF^V600E induces reversible mitotic arrest in human melanocytes via microrna-mediated suppression of AURKB.

ABSTRACT: Benign melanocytic nevi frequently emerge when an acquired BRAF^V600E mutation triggers unchecked proliferation and subsequent arrest in melanocytes. Recent observations have challenged the role of oncogene-induced senescence in melanocytic nevus formation, necessitating investigations into alternative mechanisms for the establishment and maintenance of proliferation arrest in nevi. We compared the transcriptomes of melanocytes from healthy human skin, nevi, and melanomas arising from nevi and identified a set of microRNAs as highly expressed nevus-enriched transcripts. Two of these microRNAs-MIR211-5p and MIR328-3p-induced mitotic failure, genome duplication, and proliferation arrest in human melanocytes through convergent targeting of AURKB. We demonstrate that BRAF

SUBMITTER: McNeal AS 

PROVIDER: S-EPMC8610417 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature