Single-cell transcriptome profiling reveals intratumoural heterogeneity and malignant progression in retinoblastoma.

ABSTRACT: Retinoblastoma is a childhood retinal tumour that is the most common primary malignant intraocular tumour. However, it has been challenging to identify the cell types associated with genetic complexity. Here, we performed single-cell RNA sequencing on 14,739 cells from two retinoblastoma samples to delineate the heterogeneity and the underlying mechanism of retinoblastoma progression. Using a multiresolution network-based analysis, we identified two major cell types in human retinoblastoma. Cell trajectory analysis yielded a total of 5 cell states organized into two main branches, and the cell cycle-associated cone precursors were the cells of origin of retinoblastoma that were required for initiating the differentiation and malignancy process of retinoblastoma. Tumour cells differentiatio

SUBMITTER: Yang J 

PROVIDER: S-EPMC8611004 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature