Programmable microbial ink for 3D printing of living materials produced from genetically engineered protein nanofibers.

ABSTRACT: Living cells have the capability to synthesize molecular components and precisely assemble them from the nanoscale to build macroscopic living functional architectures under ambient conditions. The emerging field of living materials has leveraged microbial engineering to produce materials for various applications but building 3D structures in arbitrary patterns and shapes has been a major challenge. Here we set out to develop a bioink, termed as "microbial ink" that is produced entirely from genetically engineered microbial cells, programmed to perform a bottom-up, hierarchical self-assembly of protein monomers into nanofibers, and further into nanofiber networks that comprise extrudable hydrogels. We further demonstrate the 3D printing of functional living materials by embedding programme

SUBMITTER: Duraj-Thatte AM 

PROVIDER: S-EPMC8611031 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature