Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation.

ABSTRACT: Lineage commitment and differentiation is driven by the concerted action of master transcriptional regulators at their target chromatin sites. Multiple efforts have characterized the key transcription factors (TFs) that determine the various hematopoietic lineages. However, the temporal interactions between individual TFs and their chromatin targets during differentiation and how these interactions dictate lineage commitment remains poorly understood. Here we perform dense, daily, temporal profiling of chromatin accessibility (DNase I-seq) and gene expression changes (total RNA-seq) along ex vivo human erythropoiesis to comprehensively define developmentally regulated DNase I hypersensitive sites (DHSs) and transcripts. We link both distal DHSs to their target gene promoters and individual

SUBMITTER: Georgolopoulos G 

PROVIDER: S-EPMC8611072 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature