Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride.

ABSTRACT: In this study, we have developed two facile spectroscopic methods for quantifying oxybutynin (OBT) hydrochloride in its pure form and tablets using design of experiments (DOEs). The spectroscopic methods depended on the ion-pair complex formation between the tertiary amino group in the drug and eosin in 0.2 M acetate buffer of pH 4. Method I involves spectrophotometric measurement of the absorbance of the developed complex at 550 nm and showed linearity through 1.0-10.0 µg ml^-1. Method II involves spectrofluorometric measurement of the quenching influence of OBT on the native fluorescence of eosin (λ excitation/λ emission of 304/548 nm) and showed linearity through 1.0-6.0 µg ml^-1. Critical parameters were identified through preliminary trials and optimized using the

SUBMITTER: Yosrey E 

PROVIDER: S-EPMC8611342 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature