Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping.

ABSTRACT:

Background

The detection and identification of single nucleotide polymorphism (SNP) is essential for determining patient disease susceptibility and the delivery of medicines targeted to the individual. At present, SNP genotyping technology includes Sanger sequencing, TaqMan-probe quantitative polymerase chain reaction (qPCR), amplification-refractory mutation system (ARMS)-PCR, and Kompetitive Allele-Specific PCR (KASP). However, these technologies have some disadvantages: the high cost of development and detection, long and time consuming protocols, and high false positive rates. Focusing on these limitations, we proposed a new SNP detection method named universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR). In this method, only two types of fluorescence-labeled pro

SUBMITTER: Li B 

PROVIDER: S-EPMC8611915 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature