Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system.

ABSTRACT: To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR-Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR-Cas reagents and challenged with BeYDV had reduced virus load and symptoms, thereby demonstrating a novel strategy for engineering resistance to geminiviruses.

SUBMITTER: Baltes NJ 

PROVIDER: S-EPMC8612103 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature