Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system.
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ABSTRACT:
SUBMITTER: Baltes NJ
PROVIDER: S-EPMC8612103 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Publications
Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system.
Baltes Nicholas J NJ Hummel Aaron W AW Konecna Eva E Cegan Radim R Bruns Aaron N AN Bisaro David M DM Voytas Daniel F DF
Nature plants 20150928 10
To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR-Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR-Cas reagents and challenged with BeYDV had reduced virus load and symp ...[more]
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