Interplay between Bacterial Clones and Plasmids in the Spread of Antibiotic Resistance Genes in the Gut: Lessons from a Temporal Study in Veal Calves.

ABSTRACT: Intestinal carriage of extended spectrum β-lactamase (ESBL)-producing Escherichia coli is a frequent, increasing, and worrying phenomenon, but little is known about the molecular scenario and the evolutionary forces at play. We screened 45 veal calves, known to have high prevalence of carriage, for ESBL-producing E. coli on 514 rectal swabs (one randomly selected colony per sample) collected over 6 months. We characterized the bacterial clones and plasmids carrying bla_ESBL genes with a combination of genotyping methods, whole genome sequencing, and conjugation assays. One hundred and seventy-three ESBL-producing E. coli isolates [bla_CTX-M-1 (64.7%), bla_CTX-M-14 (33.5%), or bla_CTX-M-15 (1.8%)] were detected, belonging t

SUBMITTER: Massot M 

PROVIDER: S-EPMC8612258 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature