Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease.

ABSTRACT: Transposition has a key role in reshaping genomes of all living organisms¹. Insertion sequences of IS200/IS605 and IS607 families² are among the simplest mobile genetic elements and contain only the genes that are required for their transposition and its regulation. These elements encode tnpA transposase, which is essential for mobilization, and often carry an accessory tnpB gene, which is dispensable for transposition. Although the role of TnpA in transposon mobilization of IS200/IS605 is well documented, the function of TnpB has remained largely unknown. It had been suggested that TnpB has a role in the regulation of transposition, although no mechanism for this has been established^3-5. A bioinformatic analysis indicated that TnpB might be a predecessor o

SUBMITTER: Karvelis T 

PROVIDER: S-EPMC8612924 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature