Cell-type specialization is encoded by specific chromatin topologies.

ABSTRACT: The three-dimensional (3D) structure of chromatin is intrinsically associated with gene regulation and cell function^1-3. Methods based on chromatin conformation capture have mapped chromatin structures in neuronal systems such as in vitro differentiated neurons, neurons isolated through fluorescence-activated cell sorting from cortical tissues pooled from different animals and from dissociated whole hippocampi^4-6. However, changes in chromatin organization captured by imaging, such as the relocation of Bdnf away from the nuclear periphery after activation⁷, are invisible with such approaches⁸. Here we developed immunoGAM, an extension of genome architecture mapping (GAM)^2,9, to map 3D chromatin topology genome-wide in specific brain ce

SUBMITTER: Winick-Ng W 

PROVIDER: S-EPMC8612935 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature