A "Failed" Assay Development for the Discovery of Rescuing Small Molecules from the Radiation Damage.

ABSTRACT: With improving survival rates for cancer patients, the side effects of radiation therapy, especially for pediatric or more sensitive adult patients, have raised interest in preventive or rescue treatment to overcome the detrimental effects of efficient radiation therapies. For the discovery of rescuing small molecules for radiation damage to the endothelium, we have been developing a 96-well microplate-based in vitro assay for high-throughput compatible measurement of radiation-induced cell damage and its rescue by phenotypic high-content imaging. In contrast to traditional radiation assays with detached cells for clonogenic formation, we observed cells with live-cell imaging in two different kinds of endothelial cells, up to three different cell densities, two gamma-infrared radiation dos

SUBMITTER: Wen KK 

PROVIDER: S-EPMC8612958 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature