Isolation and characterization of porcine macrophages and their inflammatory and fusion responses in different stiffness environments.

ABSTRACT: Evaluating the host immune response to biomaterials is an essential step in the development of medical devices and tissue engineering strategies. To aid in this process, in vitro studies, whereby immune cells such as macrophages are cultured on biomaterials, can often expedite high throughput testing of many materials prior to implantation. While most studies to date utilize murine or human cells, the use of porcine macrophages has been less well described, despite the prevalent use of porcine models in medical device and tissue engineering development. In this study, we describe the isolation and characterization of porcine bone marrow- and peripheral blood-derived macrophages, and their interactions with biomaterials. We confirmed the expression of the macrophage surface markers C

SUBMITTER: Meli VS 

PROVIDER: S-EPMC8612978 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature