Secondary electrospray ionization-high resolution mass spectrometry (SESI-HRMS) fingerprinting enabled treatment monitoring of pulmonary carcinoma cells in real time.

ABSTRACT: Lung cancer is one of the leading causes of cancer related deaths in the United States. A novel volatile analysis platform is needed to complement current diagnostic techniques and better elucidate chemical signatures of lung cancer and subsequent treatments. A systems biology bottom-up approach using cell culture volatilomics was employed to identify pathological volatile fingerprints of lung cancer in real time. An advanced secondary electrospray ionization (SESI) source, named SuperSESI was used in this study and directly attached to a Thermo Q-Exactive high-resolution mass spectrometer (HRMS). We performed a series of experiments to determine if our optimized SESI-HRMS platform can distinguish between cancer types by sampling their in vitro volatilome profiles. We detected 60 significa

SUBMITTER: Choueiry F 

PROVIDER: S-EPMC8613447 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature