Landscape of transcription termination in Arabidopsis revealed by single-molecule nascent RNA sequencing.

ABSTRACT:

Background

The dynamic process of transcription termination produces transient RNA intermediates that are difficult to distinguish from each other via short-read sequencing methods.

Results

Here, we use single-molecule nascent RNA sequencing to characterize the various forms of transient RNAs during termination at genome-wide scale in wildtype Arabidopsis and in atxrn3, fpa, and met1 mutants. Our data reveal a wide range of termination windows among genes, ranging from ~ 50 nt to over 1000 nt. We also observe efficient termination before downstream tRNA genes, suggesting that chromatin structure around the promoter region of tRNA genes may block pol II elongation. 5' Cleaved readthrough transcription in atxrn3 with delayed termination can run into downstream genes to produce

SUBMITTER: Mo W 

PROVIDER: S-EPMC8613925 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature