RNAseq analysis of treatment-dependent signaling changes during inflammation in a mouse cutaneous wound healing model.

ABSTRACT:

Background

Despite proven therapeutic effects in inflammatory conditions, the specific mechanisms of phytochemical therapies are not well understood. The transcriptome effects of Traumeel (Tr14), a multicomponent natural product, and diclofenac, a non-selective cyclooxygenase (COX) inhibitor, were compared in a mouse cutaneous wound healing model to identify both known and novel pathways for the anti-inflammatory effect of plant-derived natural products.

Methods

Skin samples from abraded mice were analyzed by single-molecule, amplification-free RNAseq transcript profiling at 7 points between 12 and 192 h after injury. Immediately after injury, the wounds were treated with either diclofenac, Tr14, or placebo control (n = 7 per group/time). RNAseq levels were compared between

SUBMITTER: St Laurent G 

PROVIDER: S-EPMC8614049 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature