PINK1 deficiency impairs osteoblast differentiation through aberrant mitochondrial homeostasis.

ABSTRACT:

Background

PTEN-induced kinase 1 (PINK1) is a serine/threonine-protein kinase in mitochondria that is critical for mitochondrial quality control. PINK1 triggers mitophagy, a selective autophagy of mitochondria, and is involved in mitochondrial regeneration. Although increments of mitochondrial biogenesis and activity are known to be crucial during differentiation, data regarding the specific role of PINK1 in osteogenic maturation and bone remodeling are limited.

Methods

We adopted an ovariectomy model in female wildtype and Pink1^-/- mice. Ovariectomized mice were analyzed using micro-CT, H&E staining, Masson's trichrome staining. RT-PCR, western blot, immunofluorescence, alkaline phosphatase, and alizarin red staining were performed to assess the expression of PIN

SUBMITTER: Lee SY 

PROVIDER: S-EPMC8614054 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature