Chemically Tuning the Antigen Release Kinetics from Spherical Nucleic Acids Maximizes Immune Stimulation.

ABSTRACT: Cancer vaccine structure is emerging as an important design factor that offers tunable parameters to enhance the targeted immune response. We report the impact of altering the antigen release rate from spherical nucleic acid (SNA) vaccines-nanoparticles with a liposomal core and surface-anchored adjuvant DNA-on immune stimulation. Peptide antigens were incorporated into SNAs using either a nonreducible linker or one of a series of reduction-triggered traceless linkers that release the native peptide at rates controlled by their substitution pattern. Compared with a nonreducible linkage, the traceless attachment of antigens resulted in lower EC₅₀ of T cell proliferation in vitro and greater dendritic cell (DC) activation and higher T cell killing ability in vivo. Tr

SUBMITTER: Skakuj K 

PROVIDER: S-EPMC8614098 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature