DNAzyme-Based Lithium-Selective Imaging Reveals Higher Lithium Accumulation in Bipolar Disorder Patient-Derived Neurons.

ABSTRACT: Lithium has been a drug for bipolar disorders (BD) for over 70 years; however, its usage has been limited by its narrow therapeutic window (between 0.6 and 1.2 mM). Understanding the cellular distribution of lithium ions (Li⁺) in patient cells will offer deep insight into this limitation, but selective imaging of Li⁺ in living cells under biomedically relevant concentration ranges has not been achieved. Herein, we report in vitro selection and development of a Li⁺-specific DNAzyme fluorescent sensor with >100-fold selectivity over other biorelevant metal ions. This sensor allows comparative Li⁺ visualization in HeLa cells, human neuronal progenitor cells (NPCs), and neurons derived from BD patients and healthy controls. Strikingly, we detected en

SUBMITTER: McGhee CE 

PROVIDER: S-EPMC8614110 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature